Application of Phase Correlation to the Montage Synthesis and 3D Reconstruction of Large Tissue Volumes from Confocal Laser Scanning Microscopy Brief title: Montage Synthesis via Phase Correlation

We have implemented and tested a new automatic method for the montage synthesis and 3D reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce “tiles” of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated in this paper.